Development of an on-line electrochemical biosensor for glucose determination in rat brain using microdialysis sampling

A biosensor for on-line monitoring of glucose in brain dialysates was devel oped. The biosensor was generated in the cross-flow amperometric cell by po tentiostatic electropolymerization of phenol (50 mM) in the presence of glu cose oxidase (935 U/mL) at + 600 mV (vs. Ag/AgCl) at a flow-rate of 5 mu L/ min at the Pt electrode. The polyphenol film ensured low interferences of a scorbate. The on-line ser-up was mathematically described and the model was used to calculate the extracellular concentration of glucose in the striat um of an anesthetized rat. The concentration of glucose was found to be 1.4 +/- 0.5 mM and 0.9 +/- 0.2 mM in two independent experiments.