Electrochemical analysis of anthramycin: Hydrolysis, DNA-interactions and quantitative determination

Authors
Teijeiro, C de la Red, E Marin, D
Citation
C. Teijeiro et al., Electrochemical analysis of anthramycin: Hydrolysis, DNA-interactions and quantitative determination, ELECTROANAL, 12(12), 2000, pp. 963-968
Citations number
21
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ELECTROANALYSIS
ISSN journal
10400397 → ACNP
Volume
12
Issue
12
Year of publication
2000
Pages
963 - 968
Database
ISI
SICI code
1040-0397(200008)12:12<963:EAOAHD>2.0.ZU;2-N
Abstract
In aqueous solution, anthramycin methyl ether (AME), anhydroanthramycin (an imino form) and anthramycin are in equilibrium. It is found that anhydroan thramycin is electrochemically active. The AME hydrolysis reaction is studi ed by cyclic voltammetry. The kinetics of the DNA-drug reaction is followed by AdTSV and it is found that the slowness of the interaction is due to an thramycin changes which take place in solution independently of the DNA pre sence. DPP is used as a sensitive method for the quantitative determination of the drug and a detection limit of 70 nM is obtained.