Cloning and sequencing analysis of TRP1 gene of Flammulina velutipes

The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencin g analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3 -glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isome rase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphat e synthase-N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding se quence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those o f basidiomycete Phanerochaete chrysosporium trpC. (C) 2000 Federation of Eu ropean Microbiological Societies. Published by Elsevier Science B.V. All ri ghts reserved.