Basidiomycetous fungus Flammulina velutipes harbors two linear mitochondrial plasmids encoding DNA and RNA polymerases

Basidiomycetous fungus Flammulina velutipes R15 strain had two linear plasm ids in its mitochondria designated pFV1 and pFV2. They were double-stranded DNAs. whose sizes were 8.3 and 8.9 kb, respectively. Sequencing analysis o f 7364 bases of the pFV1 and 6861 bases of the pFV2 revealed that the both plasmids had one set of two open reading Frames (ORFs) each of that encoded putative DNA and RNA polymerases similar to those of mitochondrial plasmid s in other filamentous fungi. In phylogenetic analysis of deduced amino aci d sequences of the ORFs and counterparts of other filamentous fungi, the pF V2 was expectedly clustered with plasmids of basidiomycetous fungi, whereas the pFV1 with kalilo plasmid of ascomycetous fungus Neurospora intermedia. (C) 2000 Federation of European Microbiological Societies. Published by El sevier Science B.V. All rights reserved.