A developmental safety study in rats using DHA- and ARA-rich single-cell oils

The long-chain omega-3 and omega-6 fatty acids, docosahexaenoic and arachid onic acids, are important in fetal development, but may be depleted from th e mother during pregnancy as she transfers reserves to the developing fetus in utero and later to the infant through her breast milli. Pregnant women can increase their dietary intake of these nutrients to maintain adequate m aternal reserves and ensure an optimal infant supply. DHASCO(R) and ARASCO( R) oils, concentrated sources of docosahexaenoic and arachidonic acids, res pectively, have been tested in acute and subchronic studies without toxic e ffects. The present developmental toxicity study was undertaken to test for potential teratogenic activity of these oils to ensure their safe use duri ng pregnancy. DHASCO and ARASCO oils were administered by oral gavage to pr egnant rats at doses up to 1250 and 2500 mg/kg body weight/day, respectivel y, during the period of organogenesis. Caesarean sections and necropsies we re performed on day 20 of gestation. Maternal reproductive outcomes were an alyzed, and fetal external, soft and skeletal tissue were examined. Treatme nt with these oils did not produce overt maternal toxicity, nor did either oil result in changes in pre- or postimplantation losses, resorptions, live births or sex ratios. Neither oil caused fetal malformations. Increased fr equencies of renal variations in development occurred in a non-dose-depende nt manner and were not toxicologically significant. We conclude that these oils are not teratogenic at doses that represent a 100-fold safety factor o ver expected use levels. (C) 2000 Elsevier Science Ltd. All rights reserved .