DISTURBANCES OF THE SECRETORY STAGE OF AMELOGENESIS IN FLUOROSED DEERTEETH - A SCANNING ELECTRON-MICROSCOPIC STUDY

Structural changes resulting from fluoride-induced disturbances of the secretory stage of amelogenesis were studied in fluorosed dental enam el of ten permanent premolars and molars from roe deer (Cervus capreol us) and red deer (Cervus elaphus). The fluorosed enamel exhibited surf ace hypoplasias of different depths and extents and an associated loss of its normal prism/interprism structure. The occurrence of such apri smatic enamel either was restricted to grossly accentuated and hypomin eralized incremental (calciotraumatic) bands or affected more extended areas to the bottom of the hypoplastic lesions. The fluoride-induced disturbance of the secretory functions of the cells had thus been eith er temporary or permanent. Layers of aprismatic enamel were regarded a s denoting periods of reduced enamel matrix formation by secretory ame loblasts lacking the distal, i.e., the prism-forming, portions of thei r Tomes processes. Our observations also indicated that the transition from the presecretory to the secretory stage of amelogenesis could be affected by fluoride, thereby preventing the ameloblasts from achievi ng their normal secretory function and from establishing fully formed Tomes processes. Aprismatic enamel was formed throughout the secretory stage of amelogenesis at these locations. The most severe ameloblast reaction that could be deduced from our findings was an abrupt cessati on of enamel matrix secretion. Some of the pathological changes observ ed in fluorosed deer enamel showed striking similarities to those repo rted in rodents after acute parenteral fluoride dosing. Thus, periods of especially elevated plasma-fluoride levels in chronically fluoride- stressed deer can cause a disruption in the function of secretory amel oblasts similar to that following acute fluoride dosing in rodents.