Analysis of the DMBT1 gene in carcinomas of the respiratory tract

Loss of chromosome 10q is a critical step during the progression and metast asis formation of lung cancer. We recently defined 3 distinct regions of al lelic imbalances and considered the DMBT1 gene at l0q25-q26 an interesting candidate for the most telomeric region. Therefore, we investigated DMBT1 i n 25 cancer cell lines and 39 primary tumors of the respiratory tract. The analysis by RT-PCR and Northern blot hybridization revealed that the gene i s expressed in all tumors and cell lines and diminished in the SCLC line H1 87, indicating that RT-PCR is critical when used as the single method for t he evaluation of gene expression. No mutations were found by SSCP analysis of the cDNA and the partially known genomic sequence. Similarly, Southern b lot hybridization was unable to detect homozygous deletions. Allelotyping o f the markers D10S587, D10S1708 and D10S1723 located near or within the DMB T1 gene did not reach the peak incidence of the 3 minimally deleted regions that we recently defined. In summary, our data do not confirm previous fin dings reporting frequent loss of DMBT1 expression in lung cancer. However. they strengthen the notion that the responsible gene on chromosome 10q25-q2 6 mediating tumor progression and metastasis formation in respiratory tract cancer remains enigmatic. (C) 2000 Wiley-Liss, Inc.