Developmental expression of phospholipase D2 mRNA in rat brain

Phospholipase D (PLD) is a ubiquitous enzyme involved in many important cel lular functions. Rat brain PLD isoforms have recently been cloned and chara cterized, but the expression of this gene has not been well studied. We thu s examined the developmental expression of PLD2 in postnatal brains by Nort hern blotting employing a non-radioactive RNA probe. Two PLD2 mRNA transcri pts of 3.9 and 10.8 kb were detected in different brain regions and various tissues of the rat. In postnatal brains, the level of PLD2 mRNA transcript s are low 1 day after birth, increase progressively during development, and reach the maximum level in the adult brain. We thus conclude that rat brai n PLD2 mRNA is developmentally regulated. (C) 2000 ISDN. Published by Elsev ier Science Ltd. All rights reserved.