In situ localization of latex allergens in 3 different brands of latex gloves by means of immunogold field emission scanning and transmission electron microscopy

Background: Latex proteins represent relevant allergens, particularly fur t hose persons who are frequently exposed to latex products (eg, health care workers and patients with chronic disorders), Although several latex allerg ens have been characterized by biochemical and molecular biologic technique s, little information is available concerning the in situ localization of a llergenic proteins in latex products. Objective: The objective of the present study was the in situ localization of latex allergens. Methods: Serum IgE from patients with latex allergy rea cting with a broad range (5-200 kd) of latex allergens was used for the in situ localization of latex allergens. One surgical and 2 examination latex slave brands were investigated by using immunogold field emission scanning and transmission electron microscopy, Results: Allergens were detected on the inner and outer surface of the glov es, particularly near the edges, crests, or folds of the bleb-like structur es visible on the surface of the latex material at high magnifications. In ultrathin cross-sections, latex allergens were Pound throughout the section s. Conclusions: Latex allergens were localized on the outer and inner surface hut also in the interior of latex gloves. The occurrence of latex allergens on the surface of latex products may be related to their potential to indu ce local reactions and, perhaps, to sensitize individuals by means of conta ct.