Adsorption of bovine serum albumin onto glass powder surfaces coated with polyvinyl alcohol

The adsorption of bovine serum albumin (BSA) was carried out onto polyvinyl alcohol-coated glass powder surfaces. The adsorbed amount was about four t imes more than that on uncoated glass. The kinetics of the adsorption proce ss was followed colorimetrically, and kinetic parameters, such as adsorptio n coefficient, rate constants for adsorption and desorption, diffusion cons tant, and penetration rate constant, were evaluated. The effects of experim ental conditions, such as pH, presence of salts, addition of aliphatic alco hols, and variation in the dielectric constant of the medium, on the amount of adsorbed BSA were investigated. The effect of temperature on adsorption was also studied, and several thermodynamic parameters, such as standard f ree energy change (Delta G degrees), heat of reaction (Delta H degrees), an d entropy change (Delta S degrees), were evaluated. (C) 2000 John Wiley & S ons, Inc.