O. Osmanagaoglu et al., Analysis of the genetic determinant for production of the pediocin P of Pediococcus pentosaecus Pep 1, J BASIC MIC, 40(4), 2000, pp. 233-241
Pediococcus pentosaceus Pep1 is a vacuum-packaged Turkish sausage isolate w
hich produces a potentially novel bacteriocin of the pediocin (anti-Listeri
a) family of peptides designated as pediocin P. Curing experiments and plas
mid profile analysis indicated that both bacteriocin immunity and productio
n determinants were linked and encoded by 9.0 MDa plasmid, pHD1.0. Attempts
to transform purified plasmid pHD1.0 into recipient Escherichia coli JM109
cells by electroporation were successful but none of the E. coli JM109 cel
ls were able to express and/or release pediocin P. However. P. pentosaceus
PC, a plasmid-cured variant of P. pentosaceus Pep1 was successfully transfo
rmed with pHD1.0 by electroporation and Bac(-)Bac(s) P. pentosaceus PC cell
s restarted to express and/or released pediocin P again as indicted by the
presence of zone of growth inhibition of L. plantarum NCDO 955 around colon
ies.