The functional unit of the renal type IIa Na+/P-i cotransporter is a monomer

The composition of the functional unit of the rat renal type IIa Na+/P-i co transporter (NaPi-IIa) was investigated by using two approaches based on th e differential sensitivities of the wild type (WT) and mutant S460C protein s to 2-aminoethyImethanethiosulfonate hydrobromide (MTSEA), a charged cyste ine modifier. Transport activity of S460C is completely blocked after incub ation in MTSEA, whereas that of the WT remains unaffected. First, Xenopus l aevis oocytes were coinjected with cRNAs coding for the WT and S460C in dif ferent proportions, and the transport inhibition after MTSEA incubation was assayed by electrophysiology. The relationship between MTSEA inhibition an d proportion of cRNA was consistent with that for a functional monomer, Sec ond, concatameric proteins were constructed that either comprised two WT pr oteins (WT-WT), two S460C mutants (S460C-S460C), or one of each (WT-S460C), Western blots of oocytes injected with fusion protein cRNA showed bands at similar to 200 kDa, whereas a main band at similar to 90 kDa was obtained for the WT cRNA alone. The kinetic properties of concatamers were the same as for the single proteins. Transport activity of the WT-WT concatamer was unaffected by MTSEA incubation, fully inhibited for S460C-S460C, but 50% in hibited for WT-S460C, This behavior was also consistent with NaPi-IIa being a functional monomer.