Lactate dehydrogenase leakage of hepatocytes with AH26 and AH plus sealer treatments

Numerous root canals filling materials are available in the field of dentis try, based on various formulas that contain a variety of different and part ly mutagenic components, such as epoxy resin sealers, Ca(OH)(2)-based mater ials, and zinc oxide-eugenol cements. AH Plus root canal sealer will not re lease formaldehyde according to the manufacturer, although AH26 does. The p urpose of this study was to analyze the leakage of lactate dehydrogenase (L DH) from rat hepatocytes after treatment with AH26 and AH Plus root canal s ealers in vitro. Hepatocytes from male Sprague-Dawley rats were used to tes t the cytotoxicity of AH26 and AH Plus. The root canal sealers weve mixed a nd then dissolved in the dimethyl sulfoxide to final concentrations of 0.01 %, 0.04%, and 0.1% (wt/vol), with a dimethyl sulfoxide concentration of <0. 05%. Dosage-dependent and time-dependent lactate dehydrogenase leakage valu es were measured and tested by one-way ANOVA. The results showed that both AH26 and AH Plus are toxic to vat hepatocytes. At a low concentration, AH26 had a higher toxicity than AH Plus to rat hepatocytes.