Relationship between copper, zinc and metallothionein in hepatocellular carcinoma and its surrounding liver parenchyma

Background/Aim: Accumulation of copper (Cu) in hepatocellular carcinoma (HC C), especially in small tumors, is greater than that in the surrounding liv er parenchyma, Metallothionein (MT) is considered to be present as Cu-MT, Z n,Cu-MT or Zn-MT. The aim of this study was to determine the presence and l ocalization of Cu-MT and Zn-MT in HCC and surrounding liver parenchyma, Methods: In 16 HCC patients, surgically resected specimens including HCC an d surrounding liver parenchyma were evaluated, Results: The level of Cu present in small HCC (<4 cm in diameter) was signi ficantly greater than that in the surrounding liver parenchyma (p<0.05), Ho wever, the level of Cu in large HCC (>4 cm in diameter) was similar to that in the surrounding liver parenchyma, Analysis by Sephadex G-75 gel filtrat ion revealed that the peak fraction due to Cu was identical to that due to MT in 14 (87.5%) of 16 HCC, the peak fraction due to Cu and Zn was identica l to that due to MT in 2 (12.5%) HCC, and the peak fraction due to Zn was i dentical to that of MT in none of 16 HCC, Conclusions: Accumulation of Cu in small HCC, in which Cu was present as Cu -MT or Zn, Cu-MT, was greater than that in the surrounding liver parenchyma , Cu accumulation and the presence of MT in the liver may be related to car cinogenesis of HCC, because of the similarity of these findings in the expe rimental data of Long-Evans rats with a cinnamon-like coat color who develo p HCC spontaneously.