Internal energies of analyte ions generated from different matrix-assistedlaser desorption/ionization matrices

Citation
E. Stevenson et al., Internal energies of analyte ions generated from different matrix-assistedlaser desorption/ionization matrices, J MASS SPEC, 35(8), 2000, pp. 1035-1041
Citations number
43
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF MASS SPECTROMETRY
ISSN journal
10765174 → ACNP
Volume
35
Issue
8
Year of publication
2000
Pages
1035 - 1041
Database
ISI
SICI code
1076-5174(200008)35:8<1035:IEOAIG>2.0.ZU;2-C
Abstract
A. transfer of energy into the internal modes of the matrix and analyte is expected to occur during matrix-assisted laser desorption/ioniziation (MALD I) processes. Both the physical and thermochemical properties of the MALDI matrix used can influence the ion internal energy and analyte ion fragmenta tion. Here we report the effect of several MALDI matrices on the relative i nternal energy of the 2'-deoxyadenylyl-(3',5')-2'-deoxyguanosine (AG) anion . Relative internal energies mere probed by low-energy collision-induced di ssociation in a Fourier transform ion cyclotron resonance mass spectrometer . Sublimation temperatures of the matrices under study were also determined and found to lie between 409 and 455 K. Analyte ion internal and initial k inetic energies did not correlate with matrix sublimation temperatures. In contrast, a strong correlation between the relative internal energy of the analyte anions and the gas-phase basicity of the matrix anions was found, T hese results suggest that gas-phase proton transfer reactions play an impor tant role in MALDI analyte ion formation and influence their internal energ y and fragmentation behavior. Copyright (C) 2000 John Wiley & Sons, Ltd.