Fm. Kuenzi et al., Reduced long-term potentiation in hippocampal slices prepared using sucrose-based artificial cerebrospinal fluid, J NEUROSC M, 100(1-2), 2000, pp. 117-122
Sucrose-based artificial cerebrospinal fluid (aCSF) is sometimes used to pr
epare brain slices for in vitro electrophysiological experiments. This stud
y compared the effect of preparing brain slices using chilled sucrose-based
aCSF versus the conventional method using chilled aCSF on hippocampal syna
ptic plasticity. Brain slices from each treatment group were transferred to
normal aCSF before electrophysiological recordings were made. The stimulus
response relationship of field excitatory postsynaptic potentials (fEPSPs)
in the CA1 region was indistinguishable between the two treatment groups.
However, the amount of LTP induced by either a theta-burst (four stimuli at
100 Hz repeated ten times at 200 ms intervals) or tetanic stimulation (100
Hz for 1 s) was significantly reduced in slices that had been prepared usi
ng sucrose-based aCSF. This was associated with reduced facilitation of the
fEPSPs during the high frequency stimulus, reduced post-tetanic potentiati
on and short-term potentiation. In sucrose-cut slices the fEPSPs: were slig
htly shorter in duration (29%, P < 0.01), and during paired-pulse stimulati
on the broadening of the second fEPSP was enhanced. The LTP deficit in sucr
ose-cut slices was reversed by blocking GABA(A) receptor. function with pic
rotoxin. These data suggest that the use of sucrose based aCSF better prese
rves GABA-mediated synaptic transmission, which limits the induction of LTP
in hippocampal brain slices. (C) 2000 Elsevier Science B.V. All rights res
erved.