A beta fibrillogenesis: Kinetic parameters for fibril formation from Congored binding

Authors
Inouye, H Kirschner, DA
Citation
H. Inouye et Da. Kirschner, A beta fibrillogenesis: Kinetic parameters for fibril formation from Congored binding, J STRUCT B, 130(2-3), 2000, pp. 123-129
Citations number
48
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STRUCTURAL BIOLOGY
ISSN journal
10478477 → ACNP
Volume
130
Issue
2-3
Year of publication
2000
Pages
123 - 129
Database
ISI
SICI code
1047-8477(200006)130:2-3<123:ABFKPF>2.0.ZU;2-Y
Abstract
Using Scatchard analysis, we have formulated as a function of time and pH t he relationship between the binding of Congo red to Alzheimer's beta-amyloi d and the aggregation number (i.e., monomer concentration within fibrils) a s defined by nucleation-dependent self-assembly. This provides a basis on w hich to determine the kinetic parameters for fibril formation from the obse rved concentration of bound Congo red. (C) 2000 Academic Press.