INDUCTION OF IP-10 CHEMOKINE PROMOTER BY MEASLES-VIRUS - COMPARISON WITH INTERFERON-GAMMA SHOWS THE USE OF THE SAME RESPONSE ELEMENT BUT WITH DIFFERENTIAL DNA-PROTEIN BINDING PROFILES

Measles virus (MV) and interferon (IFN)-gamma induced IP-10 chemokine mRNA in U373 glioblastoma cells. The minimal response element for both MV and IFN-gamma was localized between nucleotide -231 and -153 of mu IP-10 promoter, which contains an IFN-stimulated response element (ISR E) and the distal NF-kappa B-d site. Mutation of individual elements s howed that ISRE and NF-kappa B-d were required to function together. D NA-protein binding profiles with the minimal response element showed t hat IFN-gamma induced a complex consisting of STAT1 while MV induced a complex consisting of p50 and p65 in the absence of new protein synth esis. IFN-gamma and MV also induced IRF-1 DNA binding activity which p ersisted for longer time periods with IFN-gamma stimulation. Despite t he functional requirement of both ISRE and NF-kappa B-d elements, diff erent combinations of DNA binding factors are used in the induction of IP-10 by MV or IFN-gamma. (C) 1997 Elsevier Science B.V.