Characterization of the glutathione S-transferase GSTT1 deletion: discrimination of all genotypes by polymerase chain reaction indicates a trimodulargenotype-phenotype correlation

Glutathione S-transferase theta enzyme activity involved in the metabolism of toxic compounds is absent in approximately 20% of Caucasians due to a ho mozygous deletion of GSTT1 (*O/O), Because the exact manner of the GSTT1 de letion was unknown, current genotyping of GSTT1 was limited to detect the p resence versus complete absence of the gene by a GSTT1-specific polymerase chain reaction (PCR). Thus, heterozygous (*A/O) and homozygous (*A/A) sampl es could not be discriminated. We have characterized the boundaries of the deletion of the human glutathione S-transferase theta (GSTT1) gene: PCR map ping and sequencing revealed a 54251 bp fragment including GSTT1 to be dele ted from chromosome 22, most likely by a homologous recombination event bet ween two highly homologous sequence stretches that flank GSTT1, Based on th e knowledge of the GSTT1*O region, a PCR assay was devised for unambiguous discrimination of homozygously deleted (*O/O), heterozygously (*A/O) and ho mozygously GSTT1 carrying (*A/A) individuals. Genotyping of 180 samples of a Caucasian population revealed that the deletion consists of one defined a llele, whose distribution in the population fits the Hardy-Weinberg equilib rium with observed 20% *O/O, 46% *A/O and 34% *A/A individuals. The number of GSTT1*A alleles detected by this procedure correlated highly significant with the enzyme activity in erythrocytes. Genotype-phenotype comparisons d emonstrated a codominant type of inheritance by a gene-dose effect: samples with two active alleles expressed a statistically significant higher enzym atic activity compared to those with one null allele (P < 0.0001, ANOVA). P harmacogenetics 10:557-565 (C) 2000 Lippincott Williams & Wilkins.