Site-directed mutagenesis implicates a threonine residue in TM6 in the subtype selectivities of UH-AH 37 and pirenzepine at muscarinic receptors

The structural basis for the selectivity of the antagonist UH-AH 37 at huma n muscarinic acetylcholine receptors was investigated by expressing mutant receptors in COS-7 cells. Previous studies have demonstrated that the inter action between UH-AH 37 and [H-3]N-methylscopolamine in equilibrium assays is competitive and that the high affinity of UH-AH 37 for the M-5 subtype, compared to M-2, is due to an epitope in the sixth transmembrane domain (TM 6) or the third outer loop of the receptor. By mutating each nonconserved r esidue in this region of M-2 and M-5 to its counterpart in the other recept or, we identified a threonine residue in the middle of TM6 uniquely respons ible for the higher affinity of the Mg receptor (M-1, M-3, and M-4 receptor s also carry a threonine at that location and also have high affinity for U H-AH 37). The mutant receptor in which the corresponding alanine of the M-2 receptor was replaced by threonine, M(2)(401)ala double right arrow thr, e xpressed enhanced affinity for pirenzepine as well as for UH-AH 37. The chi ck M-2 receptor, which expresses anomalously high affinity for pirenzepine, differs from its mammalian counterparts by the presence of a threonine at this position. Affinities of AF-DX 116 and 4-DAMP, as well as the allosteri c potency of UH-AH 37, were not sensitive to the M-2(401) ala double right arrow thr mutation. Copyright (C) 2000 S. Karger AG, Basel.