Mutant lines of the wheat (Triticum aestivum) cultivar Hobbit 'sib', produc
ed by irradiation with fast neutrons, were evaluated for their response to
powdery mildew (Erisyphe graminis f.sp. tritici) at various growth stages t
o identify and characterize resistance. Two mutants, I3-54 and I3-48, were
found to be significantly more resistant than Hobbit 'sib', with the resist
ance being expressed in both seedlings and adult plants. Fungal hyphal deve
lopment was slower in both the mutants than in Hobbit 'sib' between 18 and
24 h after inoculation, and fewer haustoria were formed in the mutants, par
ticularly in I3-54, 72 h after inoculation. Constitutive expression of a th
aumatin-like protein gene was detected in both I3-48 and I3-54 but not in H
obbit 'sib'.