Promoter analysis of pyk20, a gene from Arabidopsis thaliana

Citation
Ps. Puzio et al., Promoter analysis of pyk20, a gene from Arabidopsis thaliana, PLANT SCI, 157(2), 2000, pp. 245-255
Citations number
34
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
157
Issue
2
Year of publication
2000
Pages
245 - 255
Database
ISI
SICI code
0168-9452(20000822)157:2<245:PAOPAG>2.0.ZU;2-Q
Abstract
The gene pyk20 which has been isolated from Arabidopsis thaliana encodes a protein with a glutamine-rich domain in the C-terminal region. The transcri ption of this gene was shown to be induced in feeding sites of root-parasit ic nematodes (Heterodera schachtii), in roots infected by a fungus-like org anism (Plasmodiophora brassicae), by plant hormone treatment, and by woundi ng. In order to identify functional promoter regions seven different 5' and 3' pyk20 promoter (ppyk20) deletion fragments were fused to the uidA gene (gus) and transformed into A. thaliana plants. Histochemical analysis of pl ants containing the different ppyk20::uidA reporter constructs was performe d during plant development in different plant tissues. Comparison of the pr omoter deletion constructs showed that the region between -277 and -1 bp is necessary to enhance the level of the GUS expression in nematode feeding s ites and by plant hormone treatment. The region between -1912 and -278 is e ssential to provide specificity of GUS expression. Conserved regulatory ele ments were identified in the ppyk20 by sequence analysis. The activation pa ttern of ppyk20 makes it well suited to engineer resistance against nematod es and other pathogens. (C) 2000 Elsevier Science Ireland Ltd. All rights r eserved.