Isolation and characterization of cytotoxic saponin chloromaloside A from Chlorophytum malayense

A cytotoxic steroidal glycoside was isolated from Chlorophytum maloyense Ri dley and its structure was characterized as a known compound, neohecogenin 3-O-beta-D-glucopyranosyl(1--> 2)-[beta-D-xylopyranosyl(1-->3)]-beta-D-gluc opyranosyl(1-->4)-beta-D-galactopyranoside (chloromaloside A). The structur al identification was performed using 2D-NMR and LC/MS/MS analysis. The pre vious, erroneously assigned H-1-NMR spectral data were revised whereas the published C-13-NMR spectral assignments were confirmed. This compound showe d in vitro cytotoxicity against several human cancer cell lines.