A. Raabe et al., Influence of dose per fraction and overall treatment time on the response of pulmonary micrometastases of the R1H-tumour to fractionated irradiation, RADIOTH ONC, 56(2), 2000, pp. 259-264
Citations number
27
Categorie Soggetti
Radiology ,Nuclear Medicine & Imaging","Onconogenesis & Cancer Research
Background and purpose: Macroscopic subcutaneously growing R1H-tumours have
been shown to respond almost independently of the dose per fraction when t
reated under ambient conditions. In addition decelerated repopulation durin
g fractionated irradiation has been shown for this experimental tumour. The
aim of the present study was to investigate whether this is also the case
for pulmonary micrometastases which are assumed to be fully oxygenated or w
hether differences in the oxygenation status of the tumour possibly alters
its response to fractionation. The influence of the dose per fraction and o
verall treatment time on the response of micrometastases to fractionated ir
radiation was studied.
Materials and methods: Pulmonary metastases were induced by i.v. injection
of viable tumour cells. Treatment was started 14 days later, when metastase
s reached an average size of four cells. Total doses of 16 to 28 Gy were ad
ministered within an overall treatment time of 11 or 25 days, using doses p
er fraction of 1, 2, or 4 Gy. Tumour response was quantified by metastatic
control (MCD37%)
Results: Fractionation had a significant influence on local control (P = 0.
009). After application of 1, 2, or 4 Gy and an overall treatment time of 1
1 days the MCD37% was 25.4 (95% C.I.: 21.5-32.0) Gy, 20.7 (17.0-24.0) Gy, a
nd 18.5 (14.9-21.6) Gy, respectively. When overall treatment time was prolo
nged to 25 days the MCD,,, increased to 25.5 (21.3-33.5) Gy when fractions
of 2 Gy where applied, but this difference was not significant (P = 0.13).
The doubling time of 12.8 days determined for the metastatic clonogenic tum
our cells during fractionated irradiation was significantly longer than the
4.1 days observed for untreated metastases (P = 0.006).
Conclusions: The results show a strong influence of fractionation on treatm
ent outcome and a decelerated repopulation during fractionated irradiation
treatment for well oxygenated pulmonary metastases of the R1H-tumour. (C) 2
000 Elsevier Science Ireland Ltd. All rights reserved.