Csb. Lopez et al., Automation of the photodegradation spectrophotometric method for the determination of diethylstilbestrol by flow injection, ANAL LETTER, 33(11), 2000, pp. 2231-2242
An spectrophotometric determination of diethylstilbestrol is proposed on th
e basis of on-line photo-degradation and measurement at 330 nm. The procedu
re is accomplished by means of flow-injection assembly including a photo-re
actor formed by a PTFE 168 cm long coil around a low-pressure mercury lamp
6 w; this photo-reactor was nested between the injection valve and the spec
trophotometric flow-cell. The sample solution was injected into a carrier s
tream of aqueous di-potassium hydrogen ortophosphate flowing at 5.0 ml min(
-1). Influence of the physic-chemical and hydrodynamic parameters was studi
ed and optimised. The calibration graph was linear up to 65 mg l(-1), and t
he procedure presented a reproducibility (rsd of the calibration slope) 1.4
%, and a sample throughput of 52 h(-1). The influence of foreign compounds
was tested.