Gaa. Ferns et al., Effect of vitamin E supplementation on circulating cell adhesion moleculespre- and post-coronary angioplasty, ANN CLIN BI, 37, 2000, pp. 649-654
The soluble adhesion molecules P-selectin (sP-selectin) and intercellular a
dhesion molecule-1 (sICAM-1) are derived from platelets and endothelial cel
ls. Circulating concentrations of these soluble adhesion molecules are rais
ed in patients with atherosclerosis and following percutaneous transluminal
coronary angioplasty (PTCA). We have investigated the effects of vitamin E
supplements (800 IU/day) on circulating plasma ICAM-1 and P-selectin level
s pre- and post-PTCA. Patients, randomized to group, were pre-treated with
vitamin E or placebo (soybean oil) for 1 month before routine PTCA. Plasma
sICAM-1 and sP-selectin were measured by enzyme-linked immunosorbent assay
on blood taken immediately pre- and post-PTCA. Total protein and alpha-toco
pherol were measured on the same samples. Plasma alpha-tocopherol concentra
tions increased in patients receiving vitamin E: 19.1 (1.5) [mean (standard
error of the mean, SEM)] mg/mL post-PTCA versus 13.9 (0.6) mg/mL pre-PTCA
(n=23; P<0.01). Plasma sP-selectin and sICAM-1 levels were not significantl
y increased following PTCA in the vitamin E group. Pre-angioplasty mean (SE
M) plasma sP-selectin concentration in the vitamin E group was 883 (0.97) n
g/mg protein; the corresponding mean post-angioplasty value was 9.34 (0.89)
ng/mg protein (P=0.85). The mean (SEM) pre-angioplasty sICAM-1 concentratio
n in this group was 2.18 (0.24) ng/mg protein, and was 2.20 (0.23) ng/mg pr
otein following angioplasty (P = 0.84). In the placebo group (n = 24) there
was a significant increase in mean (SEM) sP-selectin concentration followi
ng angioplasty, from 7.48 (0.73) to 9.70 (0.78) ng/mg protein (P<0.05). The
change (mean, SEM) in plasma sP-selectin concentration following angioplas
ty was significantly greater for the placebo group [2.22 (0.50) ng/mg prote
in] than for the group receiving vitamin E [0.50 (0.50) ng/mg protein] (P<0
.02). This difference remained significant (P<0.05) even after adjustment f
or pre-angioplasty P-selectin concentrations. Mean (SEM) plasma sICAM-1 con
centrations remained unchanged following angioplasty [pre-angioplasty: 2.16
(0.20) ng/mg protein; post-angioplasty: 1.97 (0.13) ng/mg protein]. Vitami
n E may therefore limit platelet or endothelial activation during PTCA.