Lactococcus lactis as a cell factory for high-level diacetyl production

Authors
Hugenholtz, J Kleerebezem, M Starrenburg, M Delcour, J De Vos, W Hols, P
Citation
J. Hugenholtz et al., Lactococcus lactis as a cell factory for high-level diacetyl production, APPL ENVIR, 66(9), 2000, pp. 4112-4114
Citations number
14
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
9
Year of publication
2000
Pages
4112 - 4114
Database
ISI
SICI code
0099-2240(200009)66:9<4112:LLAACF>2.0.ZU;2-9
Abstract
We report the engineering of Lactococcus lactis for the efficient conversio n of sugar into diacetyl by combining NADH-oxidase overproduction and alpha -acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diace tyl by preloading the cells with NADH-oxidase before their use as a cell fa ctory.