Micorrhization of vitroplants raised from somatic embryos of cork oak (Quercus suber L.)

The technique described herein allows in vitro ectomycorrhizal synthesis in Quercus suber vitroplants raised from somatic embryos with Pisolithus tinc torius and Scleroderma polyrhizum strains. Only strains of this species com ing from fruit bodies collected in Quercus suber stands (strain QS241 and s train QS247) formed ectomycorrhizas, and hence these species seem to exhibi t host adaptation. The in vitro mycorrhization facilitated the development of secondary roots and the ex vitro weaning of cork oak vitroplants. (C) 20 00 Elsevier Science B.V. All rights reserved.