Yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors

We have recently developed a high-efficiency method of constructing adenovi rus vectors based on Cre-mediated recombination between two plasmids co-tra nsfected into 293 cells. The simplicity and efficiency of this method shoul d greatly expedite the construction of most recombinant vectors. However th is system would not be suitable for constructing vectors bearing loxP sites elsewhere in the genome because of undesirable Cre-mediated vector rearran gements. To address this, we have developed a similar system using FLP-medi ated sites-specific recombination for the construction of adenovirus vector s.