Purification and characterization of two isoforms from Candida rugosa lipase B

Authors
Lopez, C Guerra, NP Rua, ML
Citation
C. Lopez et al., Purification and characterization of two isoforms from Candida rugosa lipase B, BIOTECH LET, 22(16), 2000, pp. 1291-1294
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
16
Year of publication
2000
Pages
1291 - 1294
Database
ISI
SICI code
0141-5492(200008)22:16<1291:PACOTI>2.0.ZU;2-U
Abstract
Two isoforms of Candida rugosa lipase B (LB1 and LB2) were purified by anio nic exchange chromatography. The lipases had the same N-terminal sequence, carbohydrate content and pH and thermal stability but different pIs and sig nificant differences in their activities against different p-nitrophenol es ters and triacylglycerides.