High-pressure induced recovery of beta-galactosidases from immunoadsorbents: stability of antigens and antibodies. Comparison with usual elution procedures

A high pressure-induced process has been developed to recover antigens from immunoadsorbents, without denaturing either the antigen, or the antibodies bound onto supports. The addition of chemicals is not needed to recover th e product. Two variants of a model antigen were considered: (i) the Escheri chia coli beta-galactosidase and (ii) a chimerical E. coli beta-galactosida se. Two and three-dimensional immunoaffinity supports (membranes and beads) were used.