Ionic strength effect on adsorption of cellobiohydrolases I and II on microcrystalline cellulose

Authors
Kim, DW Hong, YG
Citation
Dw. Kim et Yg. Hong, Ionic strength effect on adsorption of cellobiohydrolases I and II on microcrystalline cellulose, BIOTECH LET, 22(16), 2000, pp. 1337-1342
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
16
Year of publication
2000
Pages
1337 - 1342
Database
ISI
SICI code
0141-5492(200008)22:16<1337:ISEOAO>2.0.ZU;2-U
Abstract
Cellobiohydrolase I (CBH I) has a higher adsorption affinity (K-ad) and tig htness (-Delta H-a) for Avicel than cellobiohydrolase II (CBH II). The adso rption processes of CBH I and II were exothermic, and the degree of exother my were larger with the increasing ionic strength. Entropy change of CBH I was larger than CBH II with increasing ionic strength. CBH I was more effec tive than CBH II for binding at a given ionic strength.