Cloning, sequencing and heterologous expression of a new chitinase gene, chi92, from Streptomyces olivaceoviridis ATCC 11238

Authors
Li, HM Plattner, H Schimz, KL Kiess, M Diekmann, H Meens, J
Citation
Hm. Li et al., Cloning, sequencing and heterologous expression of a new chitinase gene, chi92, from Streptomyces olivaceoviridis ATCC 11238, BIOTECH LET, 22(15), 2000, pp. 1203-1209
Citations number
31
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
15
Year of publication
2000
Pages
1203 - 1209
Database
ISI
SICI code
0141-5492(200008)22:15<1203:CSAHEO>2.0.ZU;2-0
Abstract
A new chitinase gene, chi92, encoding the largest known chitinase from Stre ptomyces olivaceoviridis ATCC 11238 was sequenced by means of different PCR -methods. The cloned gene was expressed in E. coli and the recombinant prot ein could be detected by Western-blot analysis. The multiplicity of chitino lytic enzymes of this strain is discussed.