Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures

Authors
Ramirez, N Lorenzo, D Palenzuela, D Herrera, L Ayala, M Fuentes, A Perez, M Gavilondo, J Oramas, P
Citation
N. Ramirez et al., Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures, BIOTECH LET, 22(15), 2000, pp. 1233-1236
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
15
Year of publication
2000
Pages
1233 - 1236
Database
ISI
SICI code
0141-5492(200008)22:15<1233:SAFSTT>2.0.ZU;2-Z
Abstract
An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragm ent (scFv) with a 6-histidine N-terminal tag was produced in cultured trans genic tobacco cells. Western blot and antigen-specific chromatography showe d high levels of biologically active scFv in the culture supernatant (1 mg l(-1)) and in cells (5 mg kg(-1)). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.