Production of fructosylxyloside by the new fructosyltransferase from Bacillus macerans EG-6

Citation
Sw. Nam et al., Production of fructosylxyloside by the new fructosyltransferase from Bacillus macerans EG-6, BIOTECH LET, 22(15), 2000, pp. 1243-1246
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
15
Year of publication
2000
Pages
1243 - 1246
Database
ISI
SICI code
0141-5492(200008)22:15<1243:POFBTN>2.0.ZU;2-P
Abstract
The new fructosyltransferase (FTase) from Bacillus macerans EG-6 showed a b road acceptor specificity, and resulted in the formation of fructosylxylosi de (FX) with D-xylose being the most effective acceptor. The optimal FTase concentration for FX production was 0.6 unit per g sucrose, which gave the highest transfer ratio, 83%, of fructosyl moiety from sucrose to D-xylose. Maximum yield of FX was 114 g l(-1) with 200 g sucrose l(-1) and 300 g D-xy lose l(-1).