Enhancement of plasmid stability and enzymatic expression by immobilising recombinant Saccharomyces cerevisiae

Authors
Chau, TL Guillan, A Roca, E Nunez, MJ Lema, JM
Citation
Tl. Chau et al., Enhancement of plasmid stability and enzymatic expression by immobilising recombinant Saccharomyces cerevisiae, BIOTECH LET, 22(15), 2000, pp. 1247-1250
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
15
Year of publication
2000
Pages
1247 - 1250
Database
ISI
SICI code
0141-5492(200008)22:15<1247:EOPSAE>2.0.ZU;2-H
Abstract
Immobilisation of cells in a calcium alginate gel improved plasmid stabilit y (up to 50%) and enzymatic expression (up to 57%) of a recombinant Sacchar omyces cerevisiae over-expressing the homologous gene EXG1. The rate of seg regational loss in the free cells was 14-fold higher than that of the immob ilised cells. Recombinant protein synthesis requires reduced cofactors, whi ch affect the redox balance of the cell.