TFIIH is a multiprotein complex required for both transcription and DNA rep
air. Single particles of human TFIIH were revealed by electron microscopy a
nd image processing at a resolution of 3.8 nm. TFIIH is 16 x 12.5 x 7.5 nm
in size and is organized into a ring-like structure from which a large prot
ein domain protrudes out. A subcomplex assembled from five recombinant core
subunits also forms a circular architecture that can be superimposed on th
e ring found in human TFIIH. Immunolabeling experiments localize several su
bunits: p44, within the ring structure, forms the base of the protruding pr
otein density which includes the cdk7 kinase, cyclin H, and MAT1. Within th
e ring structure, p44 was flanked on either side by the XPB and XPD helicas
es. These observations provide us with a quartenary organizational model of
TFIIH.