Seven DNA polymorphisms at the candidate genes of atherosclerosis in Brazilian women with angiographically documented coronary artery disease

The possible association of genetic markers at the apolipoprotein E (HhaI p olymorphism), apolipoprotein B (XbaI, EcoRI and Ins/Del polymorphisms), and low-density lipoprotein receptor (LDLR) (AvaII, HincII and PvuII polymorph isms) with coronary artery disease (CAD) was evaluated in 50 Brazilian wome n with CAD diagnosed by angiography and in 100 healthy women (controls). Th e frequency of E3/E4 genotype for HhaI polymorphism at the Apo E gene was s ignificantly higher in CAD patients than in controls (40% vs. 14%, respecti vely, P < 0.001). Similarly, the X - X - genotype for XbaI polymorphism was more frequent in CAD individuals than controls (42% vs. 12%, P < 0.0001). The A + A + and P1P1 genotypes for AvaII and PvuII polymorphisms at the LDL R locus were also higher in CAD subjects than controls (44% vs. 16%, P < 0. 001 and 64% vs. 39%, P < 0.05, respectively). The estimated relative risks for CAD in women carrying the E3/E4, X - X -, A + A + and P1P1 genotypes we re 4.1 [95% confidence interval (CI), 3.0-5.6], 5.3 (95% CI, 3.8-7.5), 4.1 (95% CI, 3.0-5.5), and 2.8 (95% CI, 2.2-3.6), respectively. This study demo nstrates that Apo E, Apo B and LDLR gene polymorphisms are associated with CAD in Brazilian Caucasian women. (C) 2000 Elsevier Science B.V. All rights reserved.