Isolation and partial characterization of three DNA polymerases from Trypanosoma cruzi

;Three distinct DNA polymerase fractions (A, B and C), were isolated from T rypanosoma cruzi epimastigote forms. Fraction A is a low molecular mass enz yme corresponding to beta-like DNA polymerase of T. cruzi. Fraction B co-pu rified along several purification steps with fraction A, but in the last st ep it was clearly separated by a phosphocellulose chromatography. Fraction C was separated from fractions A and B by binding to DEAE-cellulose column, since the other two fractions were eluted in the flowthrough. This enzyme has an apparent native molecular mass of 100 kDa and showed it high prefere nce for poly(dC)-oligo(dG) among different template-primers tested as subst rate. Western-blot and biochemical analysis strongly suggest that the three DNA polymerase fractions correspond to different molecular entities. These results are in agreement with the idea that fraction C is a new DNA polyme rase of T. cruzi, not described before. (C) 1000 Elsevier Science Inc. All rights reserved.