Accumulation of a form of p27(Kip1) not associated with Cdk-cyclin complexes in transforming growth factor-p-arrested Mv1Lu cells

The p27(Kip1) cyclin-dependent kinase inhibitor translocates in response to transforming growth factor-beta to a Cdk2-cyclin E complex inhibiting its catalytic activity, but the p27(Kip1) protein levels are unaffected [1]. We show here that transforming growth factor-beta induces the accumulation of a form of p27(Kip1) representing a subpopulation of total p27(Kip1) in gro wth-arrested Mv1Lu epithelial cells. The inducible p27(Kip1) is detectable only by a specific p27(Kip1) monoclonal antibody recognizing a native form of p27(Kip1). The increase in this subset of p27(Kip1) correlates with G(1) arrest and withdrawal of the cells from the cycle induced by transforming growth factor-beta, serum starvation, or contact inhibition. In contrast to the majority of p27(Kip1) in the cells, the transforming growth factor-bet a-inducible p27(Kip1) is devoid of cyclin-dependent kinase/cyclin interacti ons. The results indicate that growth arresting treatments induce the accum ulation of noncyclin-dependent kinase-bound p27(Kip1), which may function a s a reservoir for inhibition of Cdk2-cyclin E activities. (C) 2000 Academic Press.