Cloning, chromosome mapping and functional characterization of a human homologue of murine Gtse-1 (B99) gene

Murine Gtse-1 (G(2) and S phase expressed protein), previously named B99, i s a wt-p53 inducible gene that encodes a microtubule-localized protein whic h is able to induce G(2)/M phase accumulation when ectopically expressed. H ere we report the cloning and characterization of a new cDNA (GTSE-1) encod ing a human homologue of the mouse Gtse-1 protein. Chromosome mapping of mo use and human genes assigned Gtse-1 to chromosome 15 and GTSE-1 to chromoso me 22q13.2-q13.3 in a region with conserved synteny to that where Gtse-1 ma pped. Analysis of the genomic structure revealed that GTSE-1 contains at le ast 11 exons and 10 introns, spanning similar to 33 kb of genomic DNA. Simi lar to murine Gtse-1, the product of GTSE-1 localized to the microtubules, was able to delay G(2)/M progression when ectopically expressed and was cel l cycle regulated. Taken together, these results indicate GTSE-1 as the hum an functional homologue of murine Gtse-1, (C) 2000 Elsevier Science B.V. Al l rights reserved.