Analysis of eight STR loci in two Hungarian populations

A multiplex reaction for the eight STR loci D3S1358, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820 was used to generate allele frequency datab ases for two Hungarian population samples, Caucasians from the Budapest are a and Romanies from Baranya county. During the analysis two intermediate-si zed alleles and a sequence variant allele were observed at the D7S820 locus . All three types of allelic variants were found to have modifications in t he same block of a (T)(9) stretch located within the 3' flanking region of each allele, which may indicate a possible higher mutation rate of this (T) (9) block. For the loci D3S1358 and D7S820 the Romany population database s howed departures from Hardy-Weinberg equilibrium. The forensic efficiency v alues for the Romany population were slightly different from those found in the Hungarian Caucasian population. Comparing the allele frequency values by G-statistic, calculating the F-ST indices and with the pair-wise compari sons of interpopulation variance, the two Hungarian populations could be di stinguished using data from the eight STR loci.