Light-induced down-regulation of the rat class 1 dynein-associated proteinrobl/LC7-like gene in visual cortex

Citation
Fc. Ye et al., Light-induced down-regulation of the rat class 1 dynein-associated proteinrobl/LC7-like gene in visual cortex, J BIOL CHEM, 275(35), 2000, pp. 27172-27176
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
275
Issue
35
Year of publication
2000
Pages
27172 - 27176
Database
ISI
SICI code
0021-9258(20000901)275:35<27172:LDOTRC>2.0.ZU;2-L
Abstract
Dynein and kinesin are the main microtubule-dependent motors that mediate i ntracellular movement in eukaryotic organisms. We have cloned a full-length cDNA encoding rat dynein light chain protein, robl/LC7-like (class 1), fro m visual cortex. We found that rat robl/LC7-like gene is highly expressed i n neocortex and displays the unusual feature of being rapidly down-regulate d by sensory stimulation. This effect was seen at both mRNA and protein lev els in visual cortex, being detectable in as little as 45 min after the ons et of visual stimulation. Down-regulation by sensory stimulation was also f ound within ocular dominance columns of area V1 in monocularly deprived mon keys. Our results suggest a high turnover rate of the robl/LC7-like protein and the presence of a repressor mechanism in neurons that is tightly coupl ed to synaptic stimulation.