Identification of CRAM, a novel unc-33 gene family protein that associateswith CRIMP3 and protein-tyrosine kinase(s) in the developing rat brain

Citation
R. Inatome et al., Identification of CRAM, a novel unc-33 gene family protein that associateswith CRIMP3 and protein-tyrosine kinase(s) in the developing rat brain, J BIOL CHEM, 275(35), 2000, pp. 27291-27302
Citations number
34
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
275
Issue
35
Year of publication
2000
Pages
27291 - 27302
Database
ISI
SICI code
0021-9258(20000901)275:35<27291:IOCANU>2.0.ZU;2-4
Abstract
Four members of collapsin response mediator proteins (CRMPs) are thought to be involved in the semaphorin-induced growth cone collapse during neural d evelopment. Here we report the identification of a novel CRMPS associated p rotein, designated CRAM for CRMP3-associated molecule, that belongs to the unc-33 gene family. The deduced amino acid sequence reveals that the CRAM g ene encodes a protein of 563 amino acids, shows 57% identity with dihydropy rimidinase, and shows 50-51% identity with CRMPs. CRAM appears to form a la rge complex composed of CRMPS and other unidentified proteins in vivo. Inde ed, CRAM physically associates with CRMPS when co-expressed in COS-7 cells. The expression of CRAM is brain-specific, is high in fetal and neonatal ra t brain, and decreases to very low levels in adult brain. Moreover, CRAM ex pression is up-regulated during neuronal differentiation of embryonal carci noma P19 and PC12 cells. Finally, immunoprecipitation analysis of rat brain extracts shows that CRAM is co-immunoprecipitated with proteins that conta in protein-tyrosine kinase activity, Taken together, our results suggest th at CRAM, which interacts with CRMP3 and protein-tyrosine kinase(s), is a ne w member of an emerging family of molecules that potentially mediate signal s involved in the guidance and outgrowth of axons.