M. Schubert et al., Bridging the gap: A set of selective H-1-N-15-correlations to link sequential neighbors of prolines, J BIOM NMR, 17(4), 2000, pp. 331-335
Triple-resonance experiments are standard in the assignment of protein spec
tra. Conventional assignment strategies use H-1-N-15-correlations as a star
ting point and therefore have problems when proline appears in the amino ac
id sequence, which lacks a signal in these correlations. Here we present a
set of amino acid selective pulse sequences which provide the information t
o link the amino acid on either side of proline residues and thus complete
the sequential assignment. The experiments yield amino acid type selective
H-1-N-15-correlations which contain signals from the amino protons of the r
esidues either preceding or following proline in the amino acid sequence. T
hese protons are correlated with their own nitrogen or with that of the pro
line. The new experiments are recorded as two-dimensional experiments and t
heir performance is demonstrated by application to a 115-residue protein do
main.