To determine the effect of different refrigeration and freezer temperatures
on the microbiological profile of chicken, 50 commercially processed broil
er chickens were each split in half on the day of processing, Equal groups
were held at 4, 0, -4, -12, and -18 degrees C (40, 32, 26, 10, and 0 degree
s F), respectively for 7 days. One half of each group was then transferred
to a 0 degrees F holding chamber for an additional 7 days. Carcass halves w
ere rinse sampled with 100 mi of phosphate-buffered saline and the diluent
sampled for mesophilic, psychrotropic, coliform, and salmonellae counts aft
er the initial 7 days at different temperatures and after 7 additional days
at -18 degrees C. Ten carcass halves were sampled on the day of processing
to give baseline counts. Mesophilic bacteria counts/ml were about log 4.6
on day 0, increased by 2 log after 7 days on carcasses held at 4 degrees C,
and were unchanged at all other storage temperatures. Psychrotropic counts
/ml were about log 3.6 on day 0 and increased during the initial 7 days by
about 3.9, 1.9, and 1.4 logs, respectively on carcasses held at 4, 0, and -
4 degrees C and had less than 1 log increase at -12 and -18 degrees C Colif
orm counts were about log 2.2/ml on day 0 and had declined to about log 1.5
/ml or less by day 7 for all storage temperatures. Escherichia coli counts/
ml were about log 2 on day 0 and were reduced about 1 log or more at other
storage days. Salmonellae counts were about log 1.5 on salmonellae-positive
carcasses and did not change appreciably at any storage temperature. No co
unts for any organism significantly changed after placement at -18 degrees
C.