Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) by polymerase chain reaction-restriction fragment length polymorphism of a 12S rRNA gene fragment

Authors
Asensio, L Gonzalez, I Fernandez, A Cespedes, A Hernandez, PE Garcia, T Martin, R
Citation
L. Asensio et al., Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) by polymerase chain reaction-restriction fragment length polymorphism of a 12S rRNA gene fragment, J FOOD PROT, 63(9), 2000, pp. 1248-1252
Citations number
21
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF FOOD PROTECTION
ISSN journal
0362028X → ACNP
Volume
63
Issue
9
Year of publication
2000
Pages
1248 - 1252
Database
ISI
SICI code
0362-028X(200009)63:9<1248:IONP(N>2.0.ZU;2-X
Abstract
Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the 12S rRNA gene has been used for the specific iden tification of Nile perch (Lares niloticus), grouper (Epinephelus guaza), an d wreck fish (Polyprion americanus). Amplification of DNA isolated from mus cle samples was carried out using a set of primers flanking a region of 436 bp from the mitochondrial 12S rRNA gene. Digestions of the PCR products wi th RsaI and Sau96I endonucleases, followed by agarose gel electrophoresis o f the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.