Marek's disease virus (MDV) homologues of herpes simplex virus type 1 UL49(VP22) and UL48 (VP16) genes: high-level expression and characterization of MDV-1 VP22 and VP16

Genes UL49 and UL48 of Marek's disease virus 1 (MDV-1) strain RB1B, encodin g the respective homologues of herpes simplex virus type 1 (HSV-1) genes VP 22 and VP16, were cloned into a baculovirus vector. Seven anti-VP22 MAbs an d one anti-VP16 MAb were generated and used to identify the tegument protei ns in cells infected lytically with MDV-1. The two genes are known to be tr anscribed as a single bicistronic transcript, and the detection of only one of the two proteins (VP22) in MSB-1 lymphoma and in chicken embryo skin ce lls infected with MDV-1 prompted the study of the transcription/translation of the UL49-48 sequence in an in vivo and in vitro expression system. VP16 was expressed in vitro at detectable levels, whereas it could only be dete cted at a lower level in a more controlled environment. It was demonstrated that VP22 is phosphorylated in insect cells and possesses the remarkable p roperty of being imported into all cells in a monolayer. VP22 localized rap idly and efficiently to nuclei, like its HSV-1 counterpart. The DNA-binding property of VP22 is also reported and a part of the region responsible for this activity was identified between aa 16 and 37 in the N-terminal region of the protein.