Estimation and isolation of azadirachtin-A from neem [Azadirachta indica A-Juss] seed kernels using high performance liquid chromatography

An improved reverse phase high performance liquid chromatography (HPLC) pro cedure has been described to estimate azadirachtin-A content in crude metha nolic extracts of neem seed kernels using valley-to-valley integration tech niques. The present paper highlights a detailed study of extraction, estima tion, and purification of azadirachtin-A present in neem seed kernels. We a lso present, herein, our results of Soxhlet extraction of Aza-A from the de fatted neem seed cake as a function of time, which is found to be more effi cient than the conventional extraction procedure.