S. Vincentnaulleau et al., STIMULATORY EFFECTS OF POLAR GLYCOPEPTIDOLIPIDS OF MYCOBACTERIUM-CHELONAE ON MURINE HEMATOPOIETIC STEM-CELLS AND MEGAKARYOCYTE PROGENITORS, Research in immunology, 148(2), 1997, pp. 127-136
The effects of polar glycopeptidolipids of Mycobacterium chelonae (pGP
L-Mc) on haematopoietic stem cells and on megakaryocyte progenitors in
bone marrow (BM) and spleen were investigated in mice. We studied the
in vivo spleen colony-forming ability and marrow repopulating ability
of pGPL-Mc by assays of colony-forming units-spleen (CFU-S), The numb
er of CFU-S was increased in BM when both donors and recipients were t
reated with pGPL-Mc. In contrast, a single treatment of donors induced
enhancement of spleen CFU-S. The number of pre-CFU-S was not signific
antly increased by pGPL-Mc injection. Megakaryocyte (Meg) progenitors
were determined in vitro with a quantitative cultural analysis of bone
marrow and spleen cells in agar in the presence of spleen-conditioned
medium. A statistically significant increase in BM and spleen CFU-Meg
was observed two days after the last administration of pGPL-Mc, This
experiment points out the ability of pGPL-Mc to induce substantial sti
mulation of megakaryocytopoiesis and slight proliferation of stem cell
s in BM, but which is more pronounced in spleen. This molecule therefo
re appears to be a potential adjuvant of chemo- and radiotherapy in or
der to palliate the cytotoxic side effects of these cancer therapeutic
modalities.